Theory and practical recommendations for autocorrelation-based image correlation spectroscopy

Theory and practical recommendations for autocorrelation-based image correlation spectroscopy.

Image correlation spectroscopy (ICS) is a powerful technique for detecting arrangement of fluorophores in images. This tutorial gives background into the mathematical underpinnings of ICS, specifically image autocorrelation. The effects of various artifacts and image processing steps, including background subtraction, noise, and image morphology were examined analytically and their effects on I...

Scanning image correlation spectroscopy.

Molecular interactions are at the origin of life. How molecules get at different locations in the cell and how they locate their partners is a major and partially unresolved question in biology that is paramount to signaling. Spatio-temporal correlations of fluctuating fluorescently tagged molecules reveal how they move, interact, and bind in the different cellular compartments. Methods based o...

Practical Course Biophysics: Fluorescence Correlation Spectroscopy

Autocorrelation and Cross- Correlation Methods

Any physical quantity that varies with time is a signal. Examples from physiology are: an electrocardiogram (ECG), an electroencephalogram (EEG), an arterial pressure waveform, and a variation of someone’s blood glucose concentration along time. In Fig. 1, one can see examples of two signals: an electromyogram (EMG) in (a) and the corresponding force in (b). When a muscle contracts, it generate...

Schwab's Practical Theory: Integrate of Theory and Practice

The emergence of re-conceptualism in the field of curriculum studies caused a large rotation of identity in this field. The book titled "understanding of curriculum" by William Pinar may be considered the most outstanding work in the field. In this book, movement which affected pre re-conceptualist, are introduced as the traditional type and it is believed the new curriculum discourses should b...